|
Aspergillus ustus was cultured in the salts media contained dextran (2%). Then the cultured liquid media were filtrated and concentrated up to 10 folds by evaporation, and then purified by means of acetone precipitation, of a repeated chromatography on the columns of DEAE-Ccellulose, Biogel P-150, and Sephadex G-200. Total proteins in the initial culture filtrate were 38,500㎎, but the final amounts of proteins were 172㎎. The specific activity of the protein in the culture filtrate was 1,340 μmoles products per minute per ㎎ protein, but the final specific activity of the protein was 2,448μ moles products per minute per ㎎ protein. The final yields remained about 30% of the initial.
|